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℡ 4000-520-616
℡ 4000-520-616
RPI/X-GAL & IPTG, Ready to Use, Non-Toxic Solution 10 mg/ml, 10 Milliliters/10 ML/B71500-0.1
产品编号:B71500-0.1
市  场 价:¥1250.00
场      地:美国(厂家直采)
产品分类: 蛋白类>聚合物>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$62.50
品      牌: rpicorp
公司分类:
RPI/X-GAL & IPTG, Ready to Use, Non-Toxic Solution 10 mg/ml, 10 Milliliters/10 ML/B71500-0.1
商品介绍
DescriptionSpecificationsDocuments

7240-90-6; 367-93-1; 872-50-4; 7732-18-5

Water

2-8°C

Stable solution of X-GAL and IPTG. Does not contain DMSO or DMF. Solution concentration is 10 mg/ml X-Gal and 8 mg/ml IPTG.Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye. Useful in Blue-White Screening Applications.Shelf Life: 1 YearWARNING! This product can expose you to 1-Methyl-2-Pyrrolidon, a chemical whichisknown to the State of California to cause developmental harm.Formoreinformationgo to www.P65Warnings.ca.gov.

0.14 lbs

2.00 x 3.50 x 1.00

Research or further manufacturing use only, not for food or drug use.
  • Appearance: Yellow clear solution
  • Sterility: Pass
  • sds_B71500_1_1.pdf
  • Download Certificate of Analysis
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